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bioD [2019-07-09 10:19:22]
function
biosynthesis of biotin
product
dethiobiotin synthase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.1|Biosynthesis/ acquisition of biotin]Gene
Coordinates
3,091,492 → 3,092,187
The protein
Catalyzed reaction/ biological activity
ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin (according to Swiss-Prot)Protein family
dethiobiotin synthetase family (according to Swiss-Prot)Structure
[PDB|1DTS] (from E. coli, 32% identity) [pubmed|8081756]Expression and Regulation
Operons
genes
[gene|C40116089BAD3F9831F8C837DC4F2E045753ABDE|bioW]-[gene|68C86FED3575B864C576DFB73FE7B5BF83994D1F|bioA]-[gene|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]-[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]-[gene|5880617EB95FC0D19219A868D6D0A3D1D0D9C532|bioB]-[gene|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|bioI]-[gene|089A32D08B5A7E552CBD9CB9745BD542863B4558|ytbQ]
description
[Pubmed|8763940,8892842]
regulatory mechanism
[protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA]: repression, [Pubmed|8763940,8892842], in [regulon|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|BirA regulon]regulation
repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|8763940,8892842]view in new tabBiological materials
Mutant
BKE30210 (Δ[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTTCCCGTCACAAAAAAAC, downstream forward: _UP4_ATGAATCAAGTGGGGGTATGBKK30210 (Δ[gene|D9A71DAF949FB6C9C22C8361D83C5A0741184977|bioD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTTCCCGTCACAAAAAAAC, downstream forward: _UP4_ATGAATCAAGTGGGGGTATGReferences
Reviews
Original publications
